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Degradation of trifloxystrobin in apples before storage

his abstract emerged as one of the outstanding research within the poster Session on Sustainable food systems and quality at the CASEE Scientific conference 2021. YPARD held a scientific competition that was tagged "Empowering young researchers of the Danube region in sustainable food systems" within the CASEE conference 2021.

The competition aimed to support the continued professional and personal growth of young professionals attending the CASEE Conference. The top three posters and the top three oral presentations related to sustainable food systems were selected. 

Enjoy reading the abstract!

Authors: S. Lazic, D. Šunjka, D. Boskovic, S. Vukovic, A. Žunić, A. Alavanja - University of Novi Sad, Serbia.

The storage of fresh apple fruits requires the application of fungicides before harvest, for the protection of causes rotting. In the controlled conditions in cold storage, fungicide residues may be stable for extended periods. For the control of the apple rot causing agent, a plant protection product based on trifloxystrobin (500 a.i./kg) was applied at the rate of 150 g/ha, with the consumption of 1000 l of water. The trial was set up at the locality Titel (Republic of Serbia), according to EPPO (2014) PP 1/018(3) method, 14 days before harvest. Apple fruit samples were collected one hour after application, and every second day for two weeks.
Samples (around 1 kg) were transferred to the laboratory and frozen to −19 °C, to avoid their decay during storage. Trifloxystrobin residues were analyzed using high-performance liquid chromatography (HPLC/DAD), with a C18 column, using mixtures of acetonitrile and acidified water as the mobile phase. Trifloxystrobin was detected and quantified at 230nm. For the extraction of trifloxystrobin, QuEChERS based method was applied. To ensure the quality of analytical results, the analytical method for the determination of trifloxystrobin residues in apple samples was validated. Obtained result for linearity (R2=0.999), recovery (99.23%), precision (RSD 0.23%) and limit of quantification (0.02mg/kg), completely fulfilled SANTE/12682/2019 criteria. The initial deposit of trifloxystrobin in apple fruits (0.33 mg/kg) was below the MRL (0.7 mg/kg). In the samples collected two and four days after the application, residues were 0.31 mg/kg and 0.22 mg/kg respectively. At the end of the pre-harvest interval of 14 days , the amount of the trifloxystrobin residues was 0.10 mg/kg. Based on these results, the calculated half-life of trifloxystrobin in apple samples was 4.33 days.

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Picture credit:  Dragana Šunjka